The undocking took place as scheduled, in an automated regime, a spokesman for Russia’s Mission Control Center said.
The spacecraft carries Roscosmos cosmonauts Pavel Vinogradov and Alexander Misurkin and NASA astronaut Christopher Cassidy, who arrived at the ISS in March.
It is scheduled to start reentry maneuvers at 6:05 Moscow time (2:05 GMT) and its landing capsule is due to touch the ground in Kazakhstan about 50 minutes later.
After the departure, the ISS crew will comprise Russian cosmonaut Fyodor Yurchikhin, and astronauts Karen Nyberg of NASA and c of the European Space Agency.
They will be joined by Russian cosmonauts Oleg Kotov and Sergei Ryazansky and NASA astronaut Michael Hopkins, who will blast off for the station in a Soyuz-TMA10M spacecraft at 00:58 Moscow time on September 26 (GMT 20:58, September 25).